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Get Price"Prolabo Microbroyeur Quantitatif Dangoumau (No. 3456c)" for two minutes. The powder was delipified in petroleum ether for 30 minutes at room temperature, further delipified in acetone (4?C) for 18 hours, and placed in a modified Raab extractor (50 g meal/200 ml acetone) for three hours at. 15?C. The delipified meal was air-dried, ground through a Wiley Mill (80-mesh sieve), and stored under
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Get Price(Dangoumau, Prolabo, Nogent-sur-Marne, France), and then stored at 2208C until analysis. Chemical analysis Chemical composition of feed was determined in accordance with the Association of Official Analytical Chemists (AOAC, 1998) procedures; NDF and ADF were determined according to the procedure of Van Soest et al. (1991). The FA of rumen fluids, substrates and residues of incu-bation
Get Pricefrozen in liquid nitrogen and ground using a Dangoumau blender (Prolabo, France). The resulting powder was extracted at 4 °C overnight with7Lofethanol/water (75/25) containing 2% of acetic acid. Ethanol/ water (75:25; v/v) was used to favor the extraction of anthocyanins over that of proanthocyanidins that are known to require solvents such as methanol or acetone/water (60:40; v/v)
Get PriceGrinding was carried out with a Dangoumau mill (Prolabo; Fig. 2), using a single ball having a vertical oscillating movement in a cylindrical pot. The pot and ball make a system linked by inertia in the absence of powder (or when the quantity of powder inside the pot is small) and submitted to sinusoidal strengths. The system has been well described by Dandurant [6]. In the case of resonance
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Get Pricenitrogen and then pulverized in a Prolabo MVicro-broyeur Quantitatif Dangoumau. Delipified meal was extracted at 4 C for 17 hr with % buffered NaCl ( g KH2PO4 and g NaHPO4/liter distilled water, pH, merthiolate at 1/10,000) at a ratio of 2 g of meal to 100 ml of buffer. The 988. September, 1970] PICKERING AND FAIRBROTHERS-SEROLOGY OF UMBELLIFERAE 989 TABLE 1. Taxa
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Get Price· Skins were immediately frozen in liquid nitrogen and ground using a Dangoumau blender (Prolabo, France). The resulting powder was sequentially extracted with ethanol/water (12:88, v/v), methanol and acetone/water (60:40, v/v), as described by Vidal et al. (2022). The acetone extract (2 l) was recovered by coarse filtration, acetone removed under vacuum at 30 °C and the extract freeze
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